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POSTERS: Plant defense response

Identification of QTL associated with resistance to root-lesion nematode (Pratylenchus neglectus) in wheat using genotyping-by-sequencing
Gurminder Singh - Department of Plant Pathology, North Dakota State University. Guiping Yan- North Dakota State University, Department of Plant Pathology, Richard Smiley- Columbia Basin Agricultural Research Center, Oregon State University, Xuehui Li- Department of Plant Sciences, North Dakota State University

The root-lesion nematode, Pratylenchus neglectus is one of the major soil-borne pathogens that reduces wheat productivity throughout the world. As very few management practices are available, utilization of host resistance is considered to be the most effective and preferred method to mitigate effects of root-lesion nematode in wheat. Phenotyping of resistance to P. neglectus in wheat is labor-intensive and time-consuming, which is the major constraint in breeding programs to develop resistant cultivars. Molecular marker assisted selection may enhance the process of developing resistant cultivars. An F2:5 recombinant inbred line population was developed from a cross between a soft white spring wheat “Louise” (susceptible parent) and an Iranian landrace “Persia 20” (resistant parent). The recombinant inbred lines were evaluated for P. neglectus resistance reactions under controlled greenhouse conditions, and then were genotyped using genotyping-by-sequencing (GBS). Using Tassel5 GBSv2 Pipeline, a total of 2,427 high-quality SNPs were identified and used to construct a linkage map. We detected one major QTL (LOD = 5.3) showing association with P. neglectus resistance, located on chromosome 4A and accounted for 35.4% of the total phenotypic variation. The SNP markers closely linked with this QTL will be converted to PCR-based markers that may provide an efficient tool to breeders for developing P. neglectus-resistant cultivars using marker-assisted selection.