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POSTERS: Proteomics / metabolomics / genomics

Mitochondrial genome of Phytophthora sansomeana, a soybean root rot pathogen
Guohong Cai - USDA-ARS / Purdue University. Steven Scofield- USDA ARS

Phytophthora sansomeana is one of two pathogens that cause Phytophthora root rot of soybean. While much molecular and genomic information is available about the other pathogen, P. sojae, only sparse information in these areas about P. sansomeana is known. We initiated an effort to sequence and annotate the genome of P. sansomeana. Here we report the assembly and annotation of its mitochondrial genome. Sequenced using PacBio technology and error-corrected using Illumina sequences, the complete mitochondrial genome was assembled into a circular molecule of 39,618 bp, with 22.03% GC content. It encodes 39 protein coding genes, 25 tRNA genes, and two ribosomal RNA genes (rnl and rns). The protein coding genes include 14 genes in the respiratory complexes (cob, cox1, cox2, cox3, nad1, nad2, nad3, nad4, nad4L, nad5, nad6, nad7, nad9, and nad11), four ATP synthase genes (atp1, atp6, atp8 and atp9), 16 ribosomal protein genes (rpl2, rpl5, rpl6, rpl14, rpl16, rps2, rps3, rps4, rps7, rps8, rps10, rps11, rps12, rps13, rps14 and rps19) and five other genes. The tRNA genes encode transfer RNAs for 19 essential amino acids except threonine. Further analysis of this mitochondria genome will be presented.