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POSTERS: Population biology and genetics

Survey of soilborne diseases in Ohio tomato high tunnels
Marlia Bosques-Martínez - The Ohio State University. Anna Testen- USDA-ARS, Christopher Taylor- The Ohio State University, Sally Miller- The Ohio State University, Department of Plant Pathology

High tunnel tomato production continues to increase every year in Ohio, however the protected environment within high tunnels creates conditions conducive for the development of soilborne disease complexes. A soilborne disease complex consisting of corky root rot (Pyrenochaeta lycopersici), black dot root rot (Colletotrichum coccodes), Verticillium wilt (Verticillium dahliae), and root knot nematode (RKN; Meloidogyne incognita and M. hapla) has become an emerging problem in tomato high tunnel production in Ohio. A survey was conducted to determine the presence and distribution of this soilborne disease complex, with the purpose of providing growers with effective management strategies. Farmers were provided with a soil sampling guide and samples were submitted to regional produce auctions or to the OARDC. A bioassay was conducted to assess the presence and severity of root rot, taproot rot and RKN galling. DNA was extracted from soil samples and species-specific primers were used to identify each pathogen. P. lycopersici was detected in 32 of 68 high tunnels (47%) and 17 of 34 farms (50%). C. coccodes was detected in 61 of 68 high tunnels (90%) and 33 of 34 farms (97%). V. dahliae was detected in 31 of 68 high tunnels (46%) and 25 of 34 farms (74%). RKN was detected in 32 of 68 high tunnels (45%) and 19 of 34 farms (56%). Growers were informed of the presence of soilborne pathogens and personalized management recommendations were given to each grower.