Since the early 1970s, Maize stripe tenuivirus (MSpV) has been reported to infect maize and other monocots around the world. MSpV was formally accepted as a species by the ICTV under the name Maize stripe virus in 1991 and finalized in 2018 as Maize stripe tenuivirus. MSpV has a multipartite genome comprised of four or five negative and ambisense RNAs. Nucleotide sequences of RNAs 2-5 were completed in the 1990s, but no complete genomic sequence of any MSpV isolate had been determined. We completed the full genomic sequences, including RNA1, of five MSpV isolates using high throughput sequencing, and partially verified them by Sanger sequencing. One isolate was from maize in Florida. Two maize and two Rottboellia cochinchinensis (itchgrass) isolates were from Papua New Guinea (PNG). Interestingly, only the isolate from FL had five RNAs. All four isolates from PNG lacked RNA5. All PNG isolates grouped together and distinct from the FL isolate in phylogenetic comparisons of RNAs 1-4. On the whole genome level nucleotide identities among the PNG isolates ranged from 97-98% for RNA 4 to 99-100% for RNA1. The nucleotide identity comparisons between each of the PNG isolates and the FL isolate was 89% for RNAs 1-2, 78% for RNA3, and 82-84% for RNA4. This research provides evidence of MSpV genomic diversity. The complete genomic sequences of multiple isolates are useful to better understand MSpV evolution and diversity and for developing diagnostic protocols.