POSTERS: Population biology and genetics
Molecular characterization of recombinant Potato virus Y (PVY) in potato from South Korea, PVYNTN strain
Alexander Karasev - University of Idaho. Kelsie Green- University of Idaho, Mariana Rodriguez-Rodriguez- University of Idaho, Joe Kuhl- University of Idaho, Mohamad Chikh Ali- University of Idaho, Sang-Min Chung- Dongguk University-Seoul, Dong-Jun Kim- Inno Seed Co.
Information about viruses affecting potato in South Korea is limited. To fill in this knowledge gap, we conducted a small survey of potato viruses in seed potato grown in South Korea. In 2017, potato plants of five cultivars exhibiting foliar mosaic, crinkling and mottle were sampled in two seed potato production areas, in Gangwon-do and Jeollabuk-do Provinces, and subjected to virus testing and characterization. Potato virus Y (PVY) was found associated with mosaic symptoms, and samples were molecularly characterized. All analyzed PVY-positive samples were found to represent the same recombinant PVY strain: PVYNTN. Three South Korean PVY isolates were subjected to whole genome sequencing using overlapping RT-PCR fragments, and all three were confirmed to represent strain PVYNTNa after a recombination analysis of the complete genomes. In phylogenetic analysis, the three South Korean isolates were placed most closely to several PVYNTNa isolates reported from Japan and Vietnam, suggesting a common source of infection. This is the first report and complete molecular characterization of a PVYNTN strain present in South Korea, and since this strain induces tuber necrotic ringspot disease in susceptible cultivars of potato, appropriate management tools need to be implemented to mitigate potential tuber quality losses.