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Poster: Diseases of Plants: Disease Detection & Diagnosis


Candidatus Liberibacter solanacearum associated to Physalis philadelphica, a new solanaceous host
H. SILVA-ROJAS (1), A. Contreras-Rendon (2), J. Sanchez-Pale (2) (1) Colegio de Postgraduados, Mexico; (2) Universidad Autonoma del Estado de Mexico, Mexico

Candidatus Liberibacter solanacearum (CaLs) bacterium is an economically important plant pathogen of solanaceous is vectored by the psyllid Bactericera cockerelli. Recently, were observed in husk tomato (Physalis philadelphica) fields in Toluca Valley in the state of Mexico, Mexico. Plants exhibiting stunting, severe leaf chlorosis, shortened internodes, curling of leaves, aborted flowers, deformation and reduced fruit size were observed, affecting the productive period. Large numbers of the psyllid B. cockerelli were observed on the crop. The objective of this research was to detect CaLs in husk tomato plants with similar symptoms as described above and whether this bacterium was present in B. cockerelli populations in the field. In 2015, we collected 250 samples from symptomatic plants and five psyliid B. cockerelli per plant in commercial plots in Toluca Valley. The molecular detection of CaLs was performed using OA2/OI2c, CLipoF/OI2c and Lp Frag 1-25F/427R primers. The amplified fragments were sequenced using Sanger methodology. Sequence alignment was carried out with the sequences belonging to the haplotypes currently recognized in GenBank database. The SNPs were identified visually. Haplotype B of CaLs was found in 100% of the plants tested, whereas in the insect found A and B haplotypes. This information will enable the approach of management strategies to husk tomato production.