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Poster: Molecular & Cellular Plant-Microbe Interactions: Proteomics/Metabolomics/Genomics


Transcriptome profiling to discover defense-related genes associated with resistance line ty-5 against Tomato yellow leaf curl virus in tomato
C. PADMABHAN (1), Y. Zheng (2), R. Shekaste-band (3), K. Stewart (1), D. Hasegawa (1), J. Scott (3), Z. Fei (4), K. Ling (1) (1) USDA-ARS, U.S. Vegetable Laboratory, U.S.A.; (2) Boyce Thompson Institute for Plant Research, U.S.A.; (3) University of Florid

Tomato yellow leaf curl virus (TYLCV), a whitefly-transmitted begomovirus, has caused serious economic losses to tomato crops in the U.S. and around the world. The most effective management would be the use of a TYLCV-resistant tomato cultivar. Several sources of TYLCV resistance genes have been identified and incorporated in tomato breeding. The ty-5 gene recently discovered in ‘Tyking’ was a recessive gene. Although its function has not been well-characterized, near-isogenic breeding materials with TYLCV resistance have been generated. In the present study, after feeding by viruliferous whiteflies (Bemisia tabaci, MEAM1) at 0, 4, 7, 14 and 21 days post inoculation (dpi), leaf materials were collected on the test tomato plants for RNA-seq. Differentially expressed (DE) genes based on genome wide transcriptome profiling were analyzed and categorized based on their functions. Those defense-related genes included pathogenesis related 1 (PR1) and 4b (PR4b), CC-NBS-LRR, defensin protein, and heat shock protein were differentially expressed in the ty-5 line. Furthermore, RNA silencing and transcriptional silencing pathway genes, such as RdRp family protein, DNA (Cytosine-5)-methyltransferase 3 and microRNA target genes were also identified. To conclude, the genome wide transcriptome analysis allows us to have a global view on the differential gene expression, which may lead us to an effective management of TYLCV through breeding for durable disease resistance cultivars.