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Poster: Molecular & Cellular Plant-Microbe Interactions: MPMI


Resistance of cultivated soybean to Clover yellow vein virus apparently originated during domestication from wild soybean
K. NAKAHARA (1), J. Abe (1), T. Yamada, (1) Y. Wang, (2), M. Hajimorad (2); Hokkaido University, Japan, (2) University of Tennessee, Knoxville, TN, USA

Clover yellow vein virus (ClYVV) (genus Potyvirus, family Potyviridae), is pathogenic to legume plants, including broad bean (Vicia faba) and pea (Pisum sativum). However, inoculation of dozen cultivated soybean (Glycine max) genotypes with ClYVV strain no30 (ClYVV-no30) did not result in systemic infection in any of the inoculated plants. In contrast, inoculation of 24 accession lines of wild soybean (G. soja) with the green fluorescent protein (GFP)-tagged ClYVV-no30 (ClYVV-no30-GFP) resulted in systemic infection in all, except one line. This observation led us to hypothesize that resistance to ClYVV in cultivated soybean originated from wild soybean during domestication. It seems likely that during soybean domestication, parental plants with natural resistance to ClYVV were selected. To test this hypothesis, and to elucidate the genetic basis of the resistance, recombinant inbred lines (RILs) were generated from a cross between cultivated soybean TK780 and wild soybean line B01167. Analysis of responses of 64 derivative RILs inoculated with ClYVV-no30-GFP resulted in identification of one major and one minor quantitative trait loci (QTL). The role of each of these loci in conferring resistance to ClYVV in cultivated soybean is being investigated.