Poster: Diseases of Plants: Disease Detection & Diagnosis
Investigation of primary infection period of apple blotch and circular leaf spot based on loop-mediated isothermal amplification assay in Korea
H. JUNG (1), S. Lee (2), S. Lee (2), J. Keum (2), S. Park (2), H. Woo (3), Y. Lim (4), I. Kang (2) (1) College of Agricultural and Life Sciences, Kyungpook National University, South Korea; (2) College of Agricultural and Life Sciences, Kyungpook National
Loop-mediated isothermal amplification (LAMP) method is widely used to detect various plant pathogens. In this study, we surveyed primary infection period of apple blotch (AB) on apple and circular leaf spot (CLS) on persimmon using developed LAMP method. The causal agent of AB and CLS; Marssonina coronaria and Mycosphaerella nawae have long incubation period to appear the typical symptoms. Up to now, primary infection period was predicted through conidia scattering period or measurement of number of conidia. To control the AB and CLS properly, primary infection periods should to be clear. Following the M. coronaria and M. nawae have long incubation period, we developed two different LAMP assay which can apply to apple and persimmon, and surveyed primary infection periods of AB and CLS in Korea using developed LAMP assay. As the results, not only infection was confirmed in healthy apple and persimmon leaves, but also the infection periods were gradually earlier than before. Although the relationship between primary infection period and fungicide program should to be studied, it is assumed that, to control and prevent economical losses of AB and CLS, fungicide spray program should be change.