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Poster: Biology & Disease Mgmt: Cultural Control


Assessment of in-vitro efficacy of different disinfectants against common bacterial diseases on woody ornamentals
P. LIYANAPATHIRANAGE (1), S. Dawadi (1), M. Kabir (2), T. Simmons (1), F. Baysal-Gurel (1) (1) Tennessee State University, U.S.A.; (2) Tennessee State University, U.S.A.

Plants are continuously exposed to disease causing agents, which enter to the nurseries in many ways. Cutting tools and other equipment that are used to work with infected plants is considered as a major source for bacterial disease spread. Various products are available in the market to use as disinfectants to clean cutting tools. This study was conducted to evaluate the effect of commercial disinfectants on Xanthomonas axonopodis, X. campestris and Pseudomonas syringae pv. syringae. We determined the contact time required to kill those bacterial pathogens by disinfectants in different chemical classes. The relative shelf-life stability of disinfectants was also determined. Replicated in-vitro experiments were conducted in which pure cultures of bacterial pathogens were exposed to disinfectants for 1, 30 and 60 sec, then 100 µL aliquots were withdrawn, plated on NBYA medium, and incubated at 25°C for 3 days. Clorox regular bleach (10%), Virkon-S (0.5, 1 and 2%), Last rinse sanitizer-beer clean, Seventh generation multi surface, Seventh generation daily shower, Seventh generation all purpose, alcohol (70%), vinegar (100%), Lysol, ZeroTol 2.0 and Green-Shield killed all three pathogens at all exposure times during 3 weeks shelf-life of products. Further studies will be done to determine efficacy of these disinfectants in preventing plant-to-plant spread of bacterial diseases through cutting tools.