Poster: Biology & Disease Mgmt: Biological Control
Investigation of antifungal and herbicide volatile terpenes in Streptomyces spp.
G. CHO (1), H. Kim (1), J. Kim (2), J. Kim (1), Y. Kwak (2) (1) Division of Applied Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea, South Korea; (2) Institute of Agriculture & Life Science, Gyeongsang National University, Jin
Streptomyces spp. produce various secondary metabolites for interaction with environment. Some of these secondary metabolites have been reported as antibiotics or volatile compounds. In addition, Streptomyces spp. have been highly expectation for discovering new secondary metabolites. We observed that mycelia of various fungi were inhibited growth when these fungi were exposed to headspace of Streptomyces spp. Furthermore, germination and growth of Arabidopsis thaliana Col-2 were inhibited significantly by these headspaces. In these headspaces, three volatile terpenes that geosmin, caryolan-1-ol and unknown terpene were discovered in common. To confirm the bioactivity, we extracted mixtures from headspace of Streptomyces griseus S4-7. Mixture included geosmin didn’t inhibit mycelia growth of Botrytis cinera. A mixture included caryolan-1-ol and unknown terpene inhibited these mycelia. Furthermore, synthetic caryolan-1-ol caused dose-dependent inhibition of these mycelia and Saccharomyces cerevisiae growth. In addition, we found three terpene cyclase gene in S. griseus S4-7 genome. Geosmin and caryolan-1-ol synthases already had been reported but the other terpene synthase was unknown. Our present results show that caryolan-1-ol was discovered new bioactivity function.