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Poster: Biology & Disease Mgmt: Biological Control


Identification of volatiles with nematicidal activities from Sphingobacterium nematocida ZY-71-1 and control efficiency to Meloidogyne incognita
J. CHANG (1), W. MU (2), J. XI (2), J. SONG (2) (1) Sanmenxia Tobacco Company Lushixian Branch, China; (2) Zhengzhou Tobacco Research Institute of CNTC, China

Chemical pesticide is an effective way in controlling tobacco disease, but it caused some problems such as environment pollution, ecological balance destruction and chemical residues. So biology control of tobacco disease attracts more and more attention. The usage of micro-organisms or microbial metabolites is an important approach in controlling nematodes. Sphingobacterium nematocida ZY-71-1?a bacterial endophyte, was isolated from tobacco leaves, which producing volatiles with nematicidal activities. The biocontrol effect of the bacterium towards Meloidogyne incognita was evaluated under Petri dish test and greenhouse assay. The mortality rate to the nematode caused by S. nematocida ZY-71-1 was 100% and the inhibition rate to the hatch of the eggs of the nematode was also 100%. Additionally, nematicidal bioactivity materials produced by S. nematocida ZY-71-1 were determined. By GC/MS analysis and pure chemicals confirmation, 5 volatiles with nematicidal activities against juveniles and eggs of M. incognita were identified from S. nematocida ZY-71-1, including benzeneacetaldehyde, 2-nonanone, decanal, 2-undecanone and dimethyl disulfide. This study provided an effective way for biological control of nematode and established foundation on controlling the nematode.