Poster: Diseases of Plants: Disease Detection & Diagnosis
PCR Assays for the Detection of Xanthomonas euvesicatoria, Causal Agent of Bacterial Spot on Pepper and Tomato Plants
R. JEONG (1), S. Son (2), M. Kyeon (2), H. Lee (3), J. Cha (2) (1) Chungbuk National University, Korea; (2) Department of Plant Medicine, Chungbuk National University, Korea; (3) Animal and Plant Quarantine Agency, Korea
Xanthomonas euvesicatoria (Xev) causes bacterial spot disease on pepper and tomato plants. A specific detection technique for this pathogen is important for the management of the disease and an efficient plant quarantine services. To obtain species-specific primers for PCR assays, low homologous ORFs of Xanthomonas euvesicatoria 85-10 to genes in NCBI GenBank were selected by BLASTn. Xev60 F/R primers that were designed from one of the select ORFs amplified specific DNA from all of tested strains of Xev including the 9 internationally collected strains and 67 Korean strains while the primers did not amplified any DNA from the 20 internationally collected strains of 3 other bacterial spot-causing Xanthomonads, X. vesicatoria, X, perforans, and X. gadneri and the other plant pathogenic bacteria. Taqman probe, Xev60-Tq was designed from the inside the PCR amplicon. Real-time PCR with Xev60 F/R and the Xev60-Tq probe generated Ct values with dose-dependent of target DNA () and Ct values of Xev strains were above of threshold Ct value for positive detection. PCR assays developed in this study can reliably detect and identify Xev.