Poster: Diseases of Plants: New & Emerging Diseases
Sequence variation between isolates of Plantago asiatica mosaic virus from lily and other hosts
J. HAMMOND (1), M. Reinsel (1) (1) USDA-ARS, USNA, Floral and Nursery Plants Research Unit, U.S.A.
Plantago asiatica mosaic virus (PlAMV) was reported from Plantago asiatica in the Russian Far East, and Nandina mosaic virus was reported from heavenly bamboo (Nandina domestica) in California, both in the 1970’s. Nandina mosaic virus has not been reported to naturally infect any other hosts and was recognized as a strain of PlAMV (PlAMV-NMV) only when the full sequence was determined. In contrast, PlAMV was reported to affect lilies (Lilium spp.) and primrose (Primula seiboldii) in Japan, and in lilies in the Netherlands, Italy, Hungary, Spain, the UK, Taiwan, Chile, and the USA. Cut-flower Oriental and Asiatic hybrid lilies suffer significant losses of foliar and flower quality, or are even rendered unmarketable by PlAMV infection, showing foliar mosaic and necrosis, and streaking or distortion of the flowers. We have sequenced the coat protein (CP) gene and 3' non-coding region of PlAMV isolates from multiple imported lily cultivars, and compared these sequences to isolates from other countries and hosts, including PlAMV-NMV. Sequences from all imported lilies fall within the same clade as PlAMV isolates from Europe; lily and primula isolates from Japan fall into another clade; whereas PlAMV-type (P. asiatica) and PlAMV-NMV form monophyletic clades. PlAMV-type and PlAMV-NMV share multiple CP amino acids differentiating them from other isolates; other residues are uniquely conserved within the ‘European’ clade, or within the ‘Japanese’ (lily and primrose) clade.