Poster: Molecular & Cellular Plant-Microbe Interactions: Proteomics/Metabolomics/Genomics
Comparative analysis of predicted proteomes from forma speciales of Fusarium oxysporum causing palm wilt
S. PONUKUMATI (1), S. Ponukumati (2), J. Huguet (2), J. Rollins (2), M. Elliott (1) (1) University of Florida, U.S.A.; (2) University of Florida, U.S.A.
Fusarium oxysporum f. sp. palmarum [FOP] and Fusarium oxysporum f. sp. canariensis [FOC] cause lethal fusarium wilt of palms in Florida. While FOC and FOP cause similar diseases, FOP has a wider host range and appears to be more aggressive than FOC. The focus of the current study is to compare the predicted FOC and FOP proteomes with various formae speciales of F. oxysporum to identify common and unique gene families. To date, the FOP and FOC genomes were sequenced, assembled and annotated using multiple bioinformatic approaches. Genome sizes of FOP and FOC are 46.9 Mbp and 46 Mbp, respectively, and the predicted proteomes consist of 15528 (FOC) and 15974 (FOP) proteins. CAZy analysis indicate FOP and FOC share similar copies of sugar transporters, cellulase, and secretory lipase, whereas FOP has additional copies of necrosis inducing proteins, cysteine rich secretory proteins and cell degrading enzymes, such as cutinase and pectate lyase. OrthoMCL was used to cluster related proteins among Fusarium genomes, resulting in 396 (FOC) and 511 (FOP) unique clusters. Secondary metabolite genes were present in 22 OrthoMCL clusters. Two gene clusters related to nonribosomal peptide synthases have increased copy number in FOP, indicating the possible role of toxins in pathogenicity. Further annotation is underway to identify putative functions of unique and common genes and to develop hypotheses concerning differences in pathogenic specificity.