ABSTRACT
Isolates of the US-1 and BR-1 clonal lineages of Phytophthora infestans, collected from tomato and potato fields of two main producing regions of Brazil (south and southeast), were tested for sensitivity to the systemic fungicide metalaxyl, plus the three protectant fungicides mancozeb, chlorothalonil, and cymoxanil. For metalaxyl, one agar test and two leaf-disc tests were carried out. For all tests, metalaxyl insensitive (I), intermediately insensitive (II), and sensitive (S) isolates were detected. There was no association among metalaxyl sensitivity and region, lineage, or host. In the agar test, 35.0% of 210 isolates were classified as I, 36.0% as II, and 29.0% as S. US-1 isolates were classified as 36.1% I, 30.6% II, and 33.3% S. BR-1 isolates were 33.3% I, 23.8% II, and 42.9% S. In leaf-disc test 1 (240 isolates tested), 24.3% were classified as I, 35.0% as II, and 40.7% as S. Isolates of US-1 were 21.0% I, 39.8% II, and 39.2% S, whereas BR-1 isolates were 36.0% I, 20.0% II, and 44.0% S. In leaf-disc test 2 (96 isolates tested), which was based on an effective dose for 50% sporulation inhibition (ED50), most isolates were either I (44.8%) or II (51.0%), and only three (4.2%) were S. The US-1 isolates were 46.0% I, 51.0% II, and 3.0% S. Isolates of the BR-1 lineage were 45.0% I, 52.0% II, and 3.0% S. For mancozeb, the ED50 for 53 of 59 isolates was below 1.0 μg/ml. No isolate grew on medium amended with more than 100 μg of chlorothalonil/ml and the ED50 for 38 of 50 isolates was below 1.0 μg/ml. For cymoxanil, the ED50 for all 47 isolates tested was below 1 μg/ml. There is no evidence of resistance of resistance of P. infestans to protectant fungicides commonly used in Brazil.
