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Electron Microscopic Detection of Novel, Coiled Viruslike Particles Associated with Graft-Inoculation of Prunus Species

Coiled, viruslike particles (spirions) were detected by electron microscopic examination of crude extracts from flowers, leaves, and/or roots of infected Prunus avium, P. mume, and P. serrulata. The particles were observed in ultrathin sections of lower epidermis, palisade, and spongy mesophyll cells of leaves of P. avium and P. mume. Spring (March and April) appears to be optimal for detection of the particles in both screenhouse and field-grown plants. The particles were successfully graft-transmitted to P. armeniaca (cvs. Luizet and Tilton), P. avium (cvs. Bing, F12/1, Mazzard, and Sam), P. mahaleb, and P. persica (cv. Elberta). Individual spirions measured 132 × 34 nm. The particles appear to be coiled forms of a filamentous virus. Filaments extending from some coiled particles were approximately 13 nm wide, with striations at a pitch of 3.24 nm. No disease symptoms or cytopathological abnormalities were associated with the presence of the particles in the Prunus species studied. The particles were not detected in virus-free control plants.