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A Flow Visualization Study of Spore Release Using a Wind Tunnel-Mounted Laser Light Sheet

September 1997 , Volume 81 , Number  9
Pages  1,057 - 1,065

J. M. Davis , Department of Marine, Earth & Atmospheric Sciences, and Department of Plant Pathology, North Carolina State University, Raleigh, 27695-8208 ; A. D. Eisner , ManTech Environmental Technology, Inc., P.O. Box 12313, Research Triangle Park, NC 27709 ; R. W. Wiener , Atmospheric Chemistry and Physics Branch, EPA National Exposure Research Laboratory, Research Triangle Park, NC 27711 ; and C. E. Main , Department of Plant Pathology, North Carolina State University, Raleigh 27695-7616



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Accepted for publication 2 June 1997.
ABSTRACT

A phase Doppler anemometry system in combination with a laser light sheet was used in a low-speed recirculating wind tunnel to examine the flow field around an individual leaf. Turbulence similar to that encountered near the surface of the earth in a neutral stability boundary layer was generated using a grid at the upwind end of the wind tunnel test section. Individual healthy and diseased plant leaves were introduced into the tunnel with the leaf tip pointing downwind. The Mie-scattered radiation from the spores departing the diseased leaf was captured on videotape. Image processing software was used to enhance the visual quality of the individual frames from the videotape and to make spore velocity calculations. Three main vortex regions around the leaf were identified. The importance of these regions to the separation of the spores from the leaf surface and their subsequent downwind movement was analyzed.


Additional keywords: coherent structures, sporangiospores

© 1997 The American Phytopathological Society