November
1997
, Volume
81
, Number
11
Pages
1,231
-
1,235
Authors
M.
Ravelonandro
,
Station de Pathologie Vegetale, INRA, Centre de Recherches de Bordeaux, BP 81 33883 Villenave d'Ornon, France
;
R.
Scorza
,
USDA-ARS Appalachian Fruit Research Station, 45 Wiltshire Road, Kearneysville, WV 25436
;
J. C.
Bachelier
,
Station de Pathologie Vegetale, INRA, Bordeaux
;
G.
Labonne
,
Laboratoire de Zoologie Agricole, ENSAM-INRA, Place Viala, 34000 Montpellier, France
;
L.
Levy
,
USDA-APHIS, PPQ, Plant Methods Development Laboratory, Bldg. 580, Beltsville, MD 20705
;
V.
Damsteegt
,
USDA-ARS Foreign Disease-Weed Science Research Unit, Fort Detrick, Frederick, MD 21702
;
A. M.
Callahan
,
USDA-ARS Appalachian Fruit Research Station, Kearneysville, WV 25436
; and
J.
Dunez
,
Station de Pathologie Vegetale, INRA, Bordeaux
Affiliations
Go to article:
RelatedArticle
Accepted for publication 29 July 1997.
Abstract
ABSTRACT
Transgenic plum trees (Prunus domestica) containing the plum pox potyvirus coat protein (PPV-CP) gene were inoculated with PPV by aphid feeding or chip budding. Infection was monitored by evaluation of virus symptoms, DAS-ELISA, and immunoblot assays. Based on observations and analyses over 3 years including two dormancy cycles, one out of five transgenic clones (C-5), was found to be resistant to infection whether inoculated by aphids or by chip budding. PPV could not be detected in any inoculated plants of the C-5 clone by immunoblot or immunocap-ture-reverse transcriptase-polymerase chain reaction assays. To our knowledge, this is the first P. domestica clone resistant to PPV infection produced by genetic engineering.
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ArticleCopyright
© 1997 The American Phytopathological Society
