Previous View
 
APSnet Home
 
Phytopathology Home


VIEW ARTICLE

Molecular Plant Pathology

Complete Nucleotide Sequences of the Infectious Cloned DNAs of Bean Dwarf Mosaic Geminivirus. Sri H. Hidayat,Department of Plant Pathology, University of Wisconsin, Madison 53706; Robert L. Gilbertson(2), Stephen F. Hanson(3), Francisco J. Morales(4), Paul Ahlquist(5), David R. Russell(6), and Douglas P. Maxwell(7). (2)(3)(7) Department of Plant Pathology, University of Wisconsin, Madison 53706, (2)Current address: Department of Plant Pathology, University of California, Davis 95616; (4)Centro Internacional de Agricultura Tropical (CIAT), Apartado Aéreo 6713, Cali, Colombia; (5)Department of Plant Pathology and Institute for Molecular Virology, University of Wisconsin, Madison 53706; (6)Agracetus, Inc., 8520 University Green, Middleton, WI 53717. Phytopathology 83:181-187. Accepted for publication 12 October 1992. Copyright 1993 The American Phytopathological Society. DOI: 10.1094/Phyto-83-181.

Bean dwarf mosaic geminivirus (BDMV) was shown to have a bipartite genome (DNA-A and DNA-B) from restriction map and sequence data comparisons of cloned DNAs. The nucleotide sequences of the infectious clones of DNA-A and DNA-B were determined. Computer analyses showed that the bipartite genome of BDMV resembles that of other whitefly-transmitted geminiviruses. The DNA-A (2,615 nt) and DNA-B (2,575 nt) have little sequence homology other than that within the common region. The nucleotide sequences of the 187-nt common regions of the two DNAs had 96% identity and included a region of 33 nt that can form a stem-loop structure nearly identical to that of other geminiviruses. Nucleotide sequence analysis identified six open reading frames, four on DNA-A and two on DNA-B, involving viral and complementary strand sense transcription. Comparisons of nucleotide and derived amino acid sequences of BDMV with those of other whitefly-transmitted geminiviruses showed that BDMV was most closely related to Abutilon mosaic geminivirus and not closely related to bean golden mosaic geminivirus. BDMV can be distinguished from other bean-infecting geminiviruses by differences in symptoms, host range, reaction of bean germplasm, and sequence identities; thus, BDMV is a distinct bean-infecting geminivirus.

Additional keywords: bean golden mosaic geminivirus, bean-infecting geminivirus, Phaseolus vulgaris, whitefly.