VIEW ARTICLE

Physiology and Biochemistry

Determination of Whole-Cell Fatty Acids in Isolates of Rhizoctonia solani AG-1 IA. Janell Stevens Johnk, Graduate research assistant, Department of Plant Pathology, University of Minnesota, St. Paul 55108; Roger K. Jones, Assistant professor, Department of Plant Pathology, University of Minnesota, St. Paul 55108. Phytopathology 82:68-72. Accepted for publication 27 July 1991. Copyright 1992 The American Phytopathological Society. DOI: 10.1094/Phyto-82-68.

The Microbial Identification System (Microbial ID, Inc., Newark, DE), used to identify and characterize fatty acids of bacteria, was modified to characterize fatty acids of isolates of Rhizoctonia solani AG-1 IA. The major fatty acid found was linoleic (18:2 cis 9, 12), which constituted 75.3–78.5% of the whole-cell fatty acid content. This fatty acid, along with palmitic (16:0) and oleic (18:1 cis 9), accounted for 95–96% of the C9–C20 fatty acids present. Smaller amounts of nine other fatty acids were consistently identified.