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Techniques

Use of Dual-Stain Fluorescence Microscopy to Observe Antagonism of Pyrenophora tritici-repentis by Limonomyces roseipellis in Wheat Straw. W. F. Pfender, Associate professor, Department of Plant Pathology, Throckmorton Hall, Kansas State University, Manhattan 66506-5502; L. G. King, and J. R. Rabe. Former research assistants, Department of Plant Pathology, Throckmorton Hall, Kansas State University, Manhattan 66506-5502. Phytopathology 81:109-112. Accepted for publication 25 July 1990. Copyright 1991 The American Phytopathological Society. DOI: 10.1094/Phyto-81-109.

A procedure was developed for differential fluorescence staining of the wheat tan spot pathogen, Pyrenophora tritici-repentis, and its antagonist, Limonomyces roseipellis. P. tritici-repentis was stained specifically by indirect immunofluorescence with the green fluorochrome FITC, and both fungi were stained red with a nonspecific lectin-conjugated fluorochrome (TRITC). Toluidine blue-O was used as a counterstain for observation of TRITC staining. In dual-stained sections of autoclaved wheat straw that was colonized by the two fungi, L. roseipellis was observed to grow in close association with and to penetrate hyphae of P. tritici-repentis.