VIEW ARTICLE

Techniques

Clean Slide Technique for the Observation of Anastomosis and Nuclear Condition of Rhizoctonia solani. W. C. Kronland, Research associate, Department of Plant Pathology, University of Arizona, Tucson 85721; M. E. Stanghellini, Professor, Department of Plant Pathology, University of Arizona, Tucson 85721. Phytopathology 78:820-822. Accepted for publication 6 January 1988. Copyright 1988 The American Phytopathological Society. DOI: 10.1094/Phyto-78-820.

A simple, rapid technique for simultaneous observation of anastomosis and nuclear condition of Rhizoctonia solani employing clean glass slides and a moist chamber is described. The use of cellophane overlays and agar-coated slides is eliminated. Anastomosis and nuclear condition can be determined in less than 24 hr.