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Postharvest Pathology and Mycotoxins

Production of a Monoclonal Antibody to T-2 Toxin with Strong Cross-Reactivity to T-2 Metabolites. E. H. Gendloff, Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824, Present address: Agrigenetics Advanced Science Company, 5649 East Buckeye Road, Madison, WI 53716; J. J. Pestka(2), D. E. Dixon(3), and L. P. Hart(4). (2)(3)Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824; (4)Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824. Phytopathology 77:57-59. Accepted for publication 30 May 1986. Copyright 1987 The American Phytopathological Society. DOI: 10.1094/Phyto-77-57.

A monoclonal antibody against T-2 toxin was produced using a mouse immunized by subcutaneous injections into the shoulder with large immunogen doses. When this antibody was used in an indirect competitive enzyme immunoassay, sensitivity to T-2 toxin was 10 ng/ml (0.5 ng per assay). The antibody cross-reacted less to HT-2 toxin than T-2 antibodies previously described. Strong cross-reaction with the T-2 metabolites 3’OH T-2 and 3’OH HT-2 was noted.