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Disease Detection and Losses

Enzyme-linked Immunosorbent Assay for Pierce’s Disease Bacteria in Plant Tissues. S. F. Nomé, Professor, Facultad de Ciencias Agropecuarias, Universidad Nacional de Cordoba, Argentina, University of California, Davis 95616; B. C. Raju(2), A. C. Goheen(3), G. Nyland(4), and D. Docampo(5). (2)(3)(4)Postgraduate research plant pathologist, research plant pathologist, United States Department of Agriculture, Science and Education Administration, and professor, respectively, Department of Plant Pathology, University of California, Davis 95616; (5)Professor, Facultad de Ciencias Agropecuarias, Universidad Nacional de Cordoba, Argentina, University of California, Davis 95616. Phytopathology 70:746-749. Accepted for publication 31 January 1980. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1980. DOI: 10.1094/Phyto-70-746.

An antiserum with a reciprocal titer of 4096 was prepared in rabbits against cultures of an isolate of Pierce’s disease bacterium from grapes. The immunoglobulin of the antiserum was purified and conjugated with alkaline phosphatase by standard procedures. The conjugate was standardized against an isolate of Pierce’s disease bacterium and used in enzyme-linked immunosorbent assay for detecting bacteria in host plant tissues. The conjugate reacted specifically to Pierce’s disease bacterium either in pure culture or host tissue, but did not react with 15 isolates of other plant pathogenic bacteria, four saprophytes, or two symbionts.