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Physiology and Biochemistry

Erysimum Latent Virus-Further Characterization as a Tymovirus. D. D. Shukla, Division of Protein Chemistry, Commonwealth Scientific and Industrial Research Organization, 343 Royal Parade, Parkville, Vic. 3052, Australia; R. Koening(2), K. H. Gough(3), W. Huth(4), and D.-E. Lesemann(5). (2)(4)(5)Institut fur Viruskrankheiten der Pflanzen, Biologische Bundesanstalt, Messeweg 11, D 3300 Braunschweig, German Federal Republic; (3)Division of Protein Chemistry, Commonwealth Scientific and Industrial Research Organization, 343 Royal Parade, Parkville, Vic. 3052, Australia. Phytopathology 70:382-384. Accepted for publication 29 October 1979. Copyright 1980 The American Phytopathological Society. DOI: 10.1094/Phyto-70-382.

Erysimum latent virus induced peripheral double membrane-bounded vesicles in chloroplasts of infected cells. Nuclei contained large masses of lightly staining shells thought to be empty shells of the virus. Electrophoresis in agarose gels at pH 7.0 caused the virus to migrate slowly towards the anode. It was a good immunogen, but serological relationships with other tymoviruses were extremely weak and found only sporadically. The capsid protein contained 208 amino acid residues determined by FITMOL analysis of the amino acid composition and had a molecular weight of 21.7 × 10³ determined by SDS polyacrylamide gel electrophoresis. The base composition of the RNA was C 34.5, A 23.9, G 15.6, U 26.0.