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Techniques

Use of Oil and Liquid Nitrogen for Quantitative Work with Helminthosporium maydis Race T. Kathleen Politowski, Graduate Research Assistant, Department of Botany and Plant Pathology, Iowa State University, Ames, IA 50011, Present address: Pfizer Genetics, Inc., P.O. Box 166, Olivia, MN 56277; Phytopathology 68:131-133. Accepted for publication 20 June 1977. Copyright © 1978 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-68-131.

Standardized inoculations with Helminthosporium maydis race T were possible over time when conidia were produced at one time, collected dry, stored in liquid nitrogen, dispensed by weight, suspended in oil, and sprayed onto plants with an aliquot inoculator.