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Physiology and Biochemistry

Infection of Barley Protoplasts with Brome Mosaic Virus. T. Okuno, Graduate Student, Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606, Japan; I. Furusawa(2), and C. Hiruki(3). (2)Professor, Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606, Japan; (3)Professor, Department of Plant Science, Faculty of Agriculture and Forestry, University of Alberta, Edmonton, Alberta, Canada, T6G 2E3. Phytopathology 67:610-615. Accepted for publication 22 November 1976. Copyright © 1977 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-67-610.

High yields of protoplasts susceptible to brome mosaic virus (BMV) were obtained by incubating barley leaves, stripped of lower epidermis, in a mixture of 0.05% Macerozyme and 2% cellulase at 30 C for 2 hr. Multiplication of BMV was demonstrated by bioassay and fluorescent antibody staining. About 30% of the protoplasts were infected under optimal conditions. Poly-L-ornithine was not essential for infection, but it stimulated both the retention of virus particles by protoplasts and the infection of protoplasts with BMV. This is the first report of virus infection of protoplasts isolated from monocotyledonous species.