VIEW ARTICLE

Comparison of Techniques for Purification of Maize Dwarf- and Sugarcane Mosaic Viruses. M. Tosic, Assistant Professor, Faculty of Agriculture, University of Belgrade, Yugoslavia; R. E. Ford(2), H. E. Moline(3), and D. E. Mayhew(4). (2)(3)(4)Professor and Research Assistants, respectively, Botany and Plant Pathology Department, Iowa State University, Ames 50010, (2)Present address: Professor and Head, Plant Pathology Dept., University of Illinois, Urbana 61801. Phytopathology 64:439-442. Accepted for publication 5 September 1973. DOI: 10.1094/Phyto-64-439.

A purification system was developed that is applicable to most known maize dwarf mosaic and sugarcane mosaic virus strains, members of the potatoe virus Y group. It incorporates acidification of pH 4.7 early in the clarification process and the use of a smaller concn of chloroform (3%) than previously reported. These viruses characteristically occur in low titer and are thus more effectively purified when the elapsed time between clarification and the final product extraction is minimized. The relative merits of this procedure and three previously published procedures are compared and discussed.