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A Medium for Heterokaryon Formation in Rhizoctonia solani. E. E. Butler, Professor, Department of Plant Pathology, University of California, Davis 95616; H. Bolkan, Graduate Student, Department of Plant Pathology, University of California, Davis 95616. Phytopathology 63:542-543. DOI: 10.1094/Phyto-63-542.

The formation of heterokaryons by Rhizoctonia solani was greatly enhanced by pairing compatible isolates on potato-dextrose agar containing 1% charcoal. On this medium, a thick, dense zone of heterokaryotic hyphae consistently formed between colonies. Thus, pairings between homokaryons, once thought to be incompatible, were found to be compatible.