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Effect of Temperature and pH on Growth in vitro of a Mycoplasmalike Organism Associated with Stubborn Disease of Citrus. Abd El-Shafy A. Fudl-Allah, Postgraduate Research Plant Pathologist, Department of Plant Pathology, University of California, Riverside 92502; E. C. Calavan, Professor of Plant Pathology, Department of Plant Pathology, University of California, Riverside 92502. Phytopathology 63:256-259. Accepted for publication 23 August 1972. DOI: 10.1094/Phyto-63-256.

A mycoplasmalike organism associated with stubborn-diseased citrus was grown in special cell-free liquid and agar media. The organism grew from pH 4 to 8, optimum pH 7, and from 9 to 39 C, optimum 30 C. Colonies formed on agar media at 9, 12, 15, 18, 21, 36, and 39 C were few and small. Larger colonies appeared at 24 to 33 C, with optimal growth at 30 C. The organism was viable after storage for 2 weeks at 4 C or for 2 months at –20 C.