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Preparation and Properties of Maize Dwarf Mosaic Virus Ribonucleic Acid. D. R. Pring, Postdoctoral Fellow, Plant Science Research Division, ARS, USDA, University of Nebraska, Lincoln 68503, Present address of senior author: Plant Virus Laboratory, University of Florida, Gainesville 32601; W. G. Langenberg, Research Plant Pathologist, Plant Science Research Division, ARS, USDA, University of Nebraska, Lincoln 68503. Phytopathology 62:253-255. Accepted for publication 8 September 1971. DOI: 10.1094/Phyto-62-253.

An infectious, single-stranded, ribonucleic acid (RNA) was prepared from purified maize dwarf mosaic virus (MDMV). The sedimentation coefficient of MDMV-RNA was about 38.9 S, as determined in linear-log sucrose density gradients. The RNA was susceptible to ribonuclease degradation and to alkali hydrolysis, resistant to deoxyribonuclease, and sedimented at 23.0 S after formaldehyde denaturation. The estimated molecular weight of MDMV-RNA is 2.7 X 10⁶.