VIEW ARTICLE

Tobacco Streak Virus in Black Raspberry. Richard H. Converse, Plant Pathologist, Plant Science Research Division, ARS, USDA, and Oregon State University, Corvallis 97331; Phytopathology 62:1001-1004. Accepted for publication 8 March 1972. DOI: 10.1094/Phyto-62-1001.

Munger black raspberry stock in Oregon is generally infected with a sap-transmissible virus serologically related to tobacco streak virus (TSV). No known disease in black raspberry is associated with this infection. The lack of cross-protection between the white clover strain of TSV and the black raspberry isolate (TSV-R), and the lack of serological reaction between TSV-R and antiserum against bean red node strain (TSV-RN), indicate that TSV-R is a distinct strain of TSV. TSV-R infected many herbaceous test plants, including cucumber from which it had a dilution end point of 1:32, a thermal end point of 56-60 C, and a longevity in vitro (5 C) of 17 days. Purification by chloroform-butanol extraction from high molarity buffered sap followed by differential and rate-zonal density-gradient centrifugations yielded a partially purified preparation with a corrected sedimentation coefficient for the heavy component (s_20,w) of 83 S. The virus particle was isometric.