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Studies on Biosynthesis of Phaseollin in Excised Pods of Phaseolus vulgaris. Samuel L. Hess, NDEA Fellow, Department of Plant Pathology, Washington State University, Pullman 99163; Lee A. Hadwiger(2), and Martin E. Schwochau(3). (2)(3)Associate Professor, and Research Associate, respectively, Department of Plant Pathology, Washington State University, Pullman 99163. Phytopathology 61:79-82. Accepted for publication 11 August 1970. DOI: 10.1094/Phyto-61-79.

Based on the incorporation of phenylalanine-ul-¹⁴C, cinnamic acid-carboxyl-¹⁴C, acetate-1-¹⁴C, and daidzein-ul-³H into phaseollin, a general biosynthetic pathway for this isoflavonoid in Phaseolus vulgaris L. is suggested. Mevalonic acid-2-¹⁴C did not appear to be incorporated, suggesting that it is not a precursor of phaseollin. Stimulation of phenylalanine ammonia-lyase (PAL) activity occurs before phaseollin can be detected in the pod tissue. The level of increased PAL activity is not always proportional to the amount of phaseollin produced.