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Improved Techniques for Initiation of Bacterial Brown Spot of Bean in the Greenhouse. S. M. Saad, Postdoctoral Fellow, Department of Plant Pathology, University of Wisconsin, Madison 53706; D. J. Hagedorn, Professor, Department of Plant Pathology, University of Wisconsin, Madison 53706. Phytopathology 61:1310-1311. DOI: 10.1094/Phyto-61-1310.

The atomizing technique for initiating bean bacterial brown spot infections in the greenhouse was superior to four other inoculation methods investigated, including pinching and rubbing with cheese-cloth, Q-tip, or pipe cleaner. Placing bean plants in a moist chamber, or covering them with plastic bags, for 24 hr before and after inoculation was not significantly better than allowing them to remain on the greenhouse bench.

Additional keywords: Phaseolus vulgaris, Pseudomonas syringae.