Previous View
 
APSnet Home
 
Phytopathology Home


VIEW ARTICLE

Inhibition of the Multiplication of Bromegrass Mosaic Virus in Barley by the Antibiotic Blasticidin S. J. Kummert, Laboratoire de Pathologie végétale, Faculté des Sciences agronomiques, 5800 Gembloux, Belgium; J. Semal, Laboratoire de Pathologie végétale, Faculté des Sciences agronomiques, 5800 Gembloux, Belgium. Phytopathology 61:10-14. Accepted for publication 3 July 1970. DOI: 10.1094/Phyto-61-10.

The effect of blasticidin S (BcS) on the multiplication of bromegrass mosaic virus (BMV) in barley was investigated. Virus synthesis was evaluated (i) by spectrophotometric measurement of the virus content of the leaves; (ii) by measuring the virus-induced RNA polymerase activity (VPA) in cell-free leaf extracts, as assayed by incorporation of tritiated uridine-5-triphosphate (UTP-3H) in the presence of actinomycin D. The action of BcS on virus-induced pathological effects was also investigated. Virus synthesis was inhibited in leaf 1, or delayed in leaf 2, when BcS was applied to one-leaf seedlings at 3 hr after inoculation with BMV; this was correlated with a similar inhibition or delay of VPA as measured in leaf extracts prepared from BcS-treated infected plants, together with a reduction of pathogenic effect. BcS added in vitro to the UTP-3H-incorporating system had no effect. It is concluded (i) that BcS acts at an early phase of infection, presumably at the level of the virus-induced RNA polymerase system; and (ii) that VPA, as measured in vitro, reflects a functional step which is a prerequisite for the in vivo synthesis of BMV.