January
2002
, Volume
92
, Number
1
Pages
65
-
74
Authors
Jean-Michel
Lett
,
Martine
Granier
,
Isabelle
Hippolyte
,
Martial
Grondin
,
Monique
Royer
,
Stéphane
Blanc
,
Bernard
Reynaud
,
and
Michel
Peterschmitt
Affiliations
First, second, and eighth authors: CIRAD-AMIS, Laboratoire de Virologie, TA 40/02, 34398 Montpellier Cedex 5, France; third author: CIRAD-AMIS, Laboratoire BIOTROP-ALBHADES, TA 40/03, 34398 Montpellier Cedex 5, France; fourth and seventh authors: CIRAD-AMIS, Pôle de Protection des Plantes, 97410 Saint-Pierre, La Réunion, France; fifth author: CIRAD-AMIS, Laboratoire IGEPAM, TA 40/02, 34398 Montpellier Cedex 5, France; and sixth author: INRA-CNRS, Laboratoire de biologie cellulaire et moléculaire, 30380 Saint-Christol les Alès, France
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RelatedArticle
Accepted for publication 10 September 2001.
Abstract
ABSTRACT
Spatial and temporal distribution of Maize streak virus (MSV, family Geminiviridae, genus Mastrevirus) was monitored in the vector species Cicadulina mbila and the nonvector species C. chinaï using conventional and real-time quantitative polymerase chain reaction. Sustained feeding on MSV-infected plants showed that virus accumulation reaches a maximum in C. chinaï, but not in C. mbila. After a 3-day acquisition access feeding period (AAP), MSV was detected in the gut, the hemolymph, and the head of C. mbila, but only in the gut of C. chinaï. Similarly, Digitaria streak virus (genus Mastrevirus), which is not transmitted by either of the two species, was only detected in the gut. MSV was detected in the hemolymph of C. mbila 3 h after the beginning of the AAP. Although viral DNA progressively decreases in the vector and nonvector species after a 3-day AAP, MSV DNA remained stable in the salivary glands of C. mbila.
JnArticleKeywords
Additional keywords:
circulative transmission,
insect compartments,
transmission barrier,
viral kinetics.
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ArticleCopyright
© 2002 The American Phytopathological Society