January
2001
, Volume
91
, Number
1
Pages
84
-
91
Authors
Benoît
Moury
,
Loïc
Cardin
,
Jean-Paul
Onesto
,
Thierry
Candresse
,
and
Alain
Poupet
Affiliations
First, second, third, and fifth authors: INRA, Unité de recherche Santé végétale et environnement, Villa Thuret, 62 bd du Cap, BP 2078, F-06606 Antibes cedex, France; and fourth author: Virologie, UMR GD2P, IBVM, INRA, BP 81, F-33883 Villenave d'Ornon cedex, France
Go to article:
RelatedArticle
Accepted for publication 20 September 2000.
Abstract
ABSTRACT
A survey for viruses in rose propagated in Europe resulted in detection of only Prunus necrotic ringspot virus (PNRSV) among seven viruses screened. Four percent of cut-flower roses from different sources were infected with PNRSV. Progression of the disease under greenhouse conditions was very slow, which should make this virus easy to eradicate through sanitary selection. Comparison of the partial coat protein gene sequences for three representative rose isolates indicated that they do not form a distinct phylogenetic group and show close relations to Prunus spp. isolates. However, a comparison of the reactivity of monoclonal antibodies raised against these isolates showed that the most prevalent PNRSV serotype in rose was different from the most prevalent serotype in Prunus spp. All of the 27 rose isolates tested infected P. persica seedlings, whereas three of the four PNRSV isolates tested from Prunus spp. were poorly infectious in Rosa indica plants. These data suggest adaptation of PNRSV isolates from Prunus spp., but not from rose, to their host plants. The test methodologies developed here to evaluate PNRSV pathogenicity in Prunus spp. and rose could also help to screen for resistant genotypes.
JnArticleKeywords
Additional keywords:
in vitro screening
,
woody plant.
Page Content
ArticleCopyright
The American Phytopathological Society, 2001