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Characterization of Mexican Isolates of Colletotrichum lindemuthianum by Using Differential Cultivars and Molecular Markers

First, second, third, fifth, seventh, and eighth authors: Department of Genetic Engineering, CINVESTAV, Unidad Irapuato, Apdo. Postal 629, Irapuato, Guanajuato, Mexico; fourth author: The Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. (INIFAP), Campo Experimental Valle del Guadiana, Apdo. Postal 186, Durango, Dgo., Mexico; and sixth author: NIFAP, Campo Experimental Valle de Mexico, Chapingo, Edo. de Mexico, Mexico

Differential cultivars and molecular markers were used to analyze 59 isolates of the bean anthracnose pathogen, Colletotrichum lindemuthianum, from different regions of Mexico. Ten distinct races were determined, three of which had not been reported previously in Mexico. Isolates were found to infect only a narrow range of the differential cultivars used and were restricted to cultivars of Middle American origin. A comparison of random amplified polymorphic DNA and amplified fragment length polymorphism (AFLP) analyses was carried out on a subset of the fungal isolates. Determination of genetic distances based on AFLP data and production of a dendrogram demonstrated two levels of association: i) isolates classified into two major groups according to the type of cultivar or system of cultivation from which they originated, and ii) isolates could be classified into smaller subgroups generally associated with the geographic location from which they were obtained. Bootstrap analysis and determination of confidence intervals showed these geographic groupings to be extremely robust.