May
2010
, Volume
23
, Number
5
Pages
608
-
617
Authors
Paolo Galli,1
Andrea Patocchi,2
Giovanni Antonio Lodovico Broggini,1 and
Cesare Gessler1
Affiliations
1Plant Pathology, Institute of Integrative Biology, ETH-Z, Universitaetstrasse 2, CH 8092 Zurich; 2Agroscope Changins-Wädenswil Research Station (ACW), Schloss, CH-8820 Wädenswil, Switzerland
Go to article:
RelatedArticle
Accepted 23 December 2009.
Abstract
Scab caused by the pathogen Venturia inaequalis is considered the most important fungal disease of cultivated apple (Malus × domestica Borkh.). In all, 16 monogenic resistances against scab have been found in different Malus spp. and some of them are currently used in apple breeding for scab-resistant cultivars. However, the self incompatibility and the long generation time of Malus spp. together with the high standards of fruit quality demanded from the fresh market render the breeding of high-quality cultivars in apple a long and expensive task. Therefore, the cloning of disease resistance genes and the use of the cloned genes for the transformation of high-quality apple cultivars could be an approach to solve these drawbacks. We report the construction of a bacterial artificial chromosome (BAC) contig spanning the Rvi15 (Vr2) apple scab resistance locus using two GMAL 2473 BAC libraries. A single BAC clone of the contig was sufficient to span the resistance locus. The BAC clone was completely sequenced, allowing identification of a sequence of 48.6 kb going from the two closest markers (ARGH17 and 77G20RP) bracketing Rvi15 (Vr2). Analysis of the 48.6-kb sequence revealed the presence of three putative genes characterized by a Toll and mammalian interleukin-1 receptor protein nucleotide-binding site leucine-rich repeat structure. All three genes were found to be transcribed.
JnArticleKeywords
Page Content
ArticleCopyright
© 2010 The American Phytopathological Society