September
2007
, Volume
20
, Number
9
Pages
1,112
-
1,125
Authors
Dale Godfrey,1
Amanda J. Able,2 and
Ian B. Dry1
Affiliations
1CSIRO Plant Industry, PO Box 350, Glen Osmond, SA 5064, Australia; 2Plant and Food Science, School of Agriculture, Food & Wine, The University of Adelaide, Waite Campus, PMB1, Glen Osmond, SA 5064, Australia
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RelatedArticle
Accepted 4 May 2007.
Abstract
Germin-like proteins (GLP) have various proposed roles in plant development and defense. Seven novel GLP cDNA clones were isolated from grapevine (Vitis vinifera cv. Chardonnay). Reverse transcriptase-polymerase chain reaction expression analysis revealed that the VvGLP genes exhibit diverse and highly specific patterns of expression in response to a variety of abiotic and biotic treatments, including challenge by Erysiphe necator, Plasmopara viticola, and Botrytis cinerea, suggesting a diversity of roles for each of the GLP family members. Significantly, one of the grapevine GLP genes, VvGLP3, is induced specifically by E. necator infection and expression is closely linked to the site of infection. Subcellular localization of VvGLP3 determined by transient expression of a VvGLP3:GFP fusion construct in onion cells indicated that the recombinant protein was targeted to the cell wall. Recombinant VvGLP3 was successfully expressed in Arabidopsis thaliana and the partially purified recombinant protein was demonstrated to have superoxide dismutase activity. This data has provided an insight into the diverse nature of the GLP family in grapevine and suggests that VvGLP3 may be involved in the defense response against E. necator.
JnArticleKeywords
Additional keywords:
epidermal,
powdery mildew.
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ArticleCopyright
© 2007 The American Phytopathological Society