May
2005
, Volume
18
, Number
5
Pages
375
-
384
Authors
Lijuan
Wang
,
1
,
2
Zhongyou
Pei
,
1
Yingchuan
Tian
,
1
and
Chaozu
He
1
,
3
Affiliations
1National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P. R. China; 2Graduate School of the Chinese Academy of Sciences, Beijing 100039, P. R. China; 3Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, P. R. China
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Accepted 30 November 2004.
Abstract
The Arabidopsis LSD1 and LOL1 proteins both contain three conserved zinc finger domains and have antagonistic effects on plant programmed cell death (PCD). In this study, a rice (Oryza sativa) functional homolog of LSD1, designated OsLSD1, was identified. The expression of OsLSD1 was light-induced or dark-suppressed. Overexpression of OsLSD1 driven by the cauliflower mosaic virus 35S promoter accelerated callus differentiation in transformed rice tissues and increased chlorophyll b content in transgenic rice plants. Antisense transgenic rice plants exhibited lesion mimic phenotype, increased expression of PR-1mRNA, and an accelerated hypersensitive response when inoculated with avirulent isolates of blast fungus. Both sense and antisense transgenic rice plants conferred significantly enhanced resistance against a virulent isolate of blast fungus. Moreover, ectopic overexpression of OsLSD1 in transgenic tobacco (Nicotiana tabacum) enhanced the tolerance to fumonisins B1 (FB1), a PCD-eliciting toxin. OsLSD1 green fluorescent protein fusion protein was located in the nucleus of tobacco cells. Our results suggest that OsLSD1 plays a negative role in regulating plant PCD, whereas it plays a positive role in callus differentiation.
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© 2005 The American Phytopathological Society