April
2004
, Volume
17
, Number
4
Pages
374
-
382
Authors
Sanjay Kumar
Jain
,
1
Gregor
Langen
,
1
Wolfgang
Hess
,
2
Thomas
Börner
,
2
Ralph
Hückelhoven
,
1
and
Karl-Heinz
Kogel
1
Affiliations
1Interdisciplinary Research Centre for Environmental Sciences, Institute of Phytopathology and Applied Zoology, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26, D-35392 Giessen, Germany; 2Department of Biology, Humboldt-University Berlin, Chausseestr. 117, D-10115 Berlin, Germany
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RelatedArticle
Accepted 24 November 2003.
Abstract
We performed cytological and molecular analyses of the interaction between the biotrophic barley powdery mildew fungus Blumeria graminis f. sp. hordei and white and green leaves of the barley albostrians mutant. The leaves have the same nuclear genotype but differ from each other in respect to plastid differentiation. White leaves showed enhanced penetration resistance to B. graminis f. sp. hordei, associated with higher epidermal H2O2 accumulation beneath the appressorial germ tubes and protein cross-linking in papillae. Very low basal salicylic acid content was found in white leaves, which further confirmed that H2O2 accumulation and penetration resistance in barley are independent of salicylic acid. Expression analysis of stress and defense-related genes, including such being involved in reactive oxygen species production and cell death regulation, revealed stronger constitutive or pathogen-induced transcript accumulation in white leaves. We discuss the data on the basis of the finding that white albostrians leaves exhibit a supersusceptible interaction phenotype with the hemibiotrophic fungus Bipolaris sorokiniana.
JnArticleKeywords
Additional keywords:
GDP-dissociation inhibitor,
MLO,
OXLP.
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© 2004 The American Phytopathological Society