January
2002
, Volume
15
, Number
1
Pages
35
-
42
Authors
Bas F.
Brandwagt
,
Tarcies J. A.
Kneppers
,
H. John J.
Nijkamp
,
and
Jacques
Hille
Affiliations
Department of Genetics, Free University, Institute for Molecular Biological Sciences, BioCentrum Amsterdam, De Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands
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RelatedArticle
Accepted 5 September 2001.
Abstract
The sphinganine-analog mycotoxins (SAMs) fumonisin B1 and AAL toxins are inhibitors of eukaryotic sphinganine N-acyltransferase in vitro. Treatment of eukaryotes with SAMs generally results in an accumulation of sphingoid base precursors and a depletion of complex sphingolipids. The asc,asc genotypes of tomato (Lycopersicon esculentum) and Nicotiana umbratica are sensitive to SAMs and host of the AAL toxin-producing fungus Alternaria alternata f. sp. lycopersici. Codominant insensitivity to SAMs in tomato is mediated by the Asc-1 gene, and sensitivity is associated with a frame-shift mutation present in asc-1. We investigated the function of Asc-1 in mediating insensitivity to SAMs and resistance to the fungus by overexpression of asc-1 and Asc-1. In this study, it is shown that overexpression of these genes did not lead to visual symptoms in tomato hairy roots and N. umbratica plants. Overexpression of asc-1 did not influence the (in)sensitivity to SAMs. Overexpression of Asc-1 in SAM-sensitive hairy roots and N. umbratica plants, however, mediated a high insensitivity to SAMs and resistance to plant infection by Alternaria alternata f. sp. lycopersici.
JnArticleKeywords
Additional keywords:
apoptosis
,
ceramide
,
Fusarium moniliforme
,
LAG1
,
stem canker.
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ArticleCopyright
© 2002 The American Phytopathological Society