July
2000
, Volume
13
, Number
7
Pages
787
-
790
Authors
Kai
Lemcke
,
1
Els
Prinsen
,
2
Harry
van Onckelen
,
2
and
Thomas
Schmülling
1
Affiliations
1Zentrum für Molekularbiologie der Pflanzen (ZMBP), Allgemeine Genetik, University of Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany; 2University of Antwerp, Department of Biology, B-2610 Antwerp, Belgium
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RelatedArticle
Accepted 23 March 2000.
Abstract
The open reading frame 8 (ORF8) is located on the TL-DNA of the phytopathogenic soil bacterium Agrobacterium rhizogenes strain A4. The predicted ORF8 protein has a particular structure and is possibly a natural fusion protein. The N-terminal domain shows homology to the A. rhizogenes rolB protein and may modulate the auxin responsiveness of host cells. The C terminus has up to 38% homology to tryptophan 2-monooxygenases (t2m). We show that ORF8 overexpressing plants contain a fivefold higher concentration of indole-3-acetamide (IAM) than untransformed plants. Protein extracts from seedlings and Escherichia coli overexpressing ORF8 show significantly higher turnover rates of tryptophan to IAM than negative controls. We conclude that the ORF8 gene product has tryptophan 2-monooxygenase activity.
JnArticleKeywords
Additional keywords:
aux1 gene,
auxin sensitivity,
T-DNA.
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ArticleCopyright
© 2000 The American Phytopathological Society