We studied the response of the actin cytoskeleton in vetch root hairs after application of host-specific Nod factor. Within 3 to 15 min, the number of sub-apical fine bundles of actin filaments (FB-actin) increased in all developmental stages. Tip growth resumed only in hairs in which the FB-actin density and the length of the region with FB-actin exceeded a minimal value.
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