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Host Range Limitation of a Pseudorecombinant Virus Produced by Two Distinct Bipartite Geminiviruses

November 1997 , Volume 10 , Number  8
Pages  1,019 - 1,022

Petra Höfer , Margit Engel , Holger Jeske , and Thomas Frischmuth

Biologisches Institut, Lehrstuhl für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, 70550 Stuttgart, Germany


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Accepted 5 August 1997.

The production of pseudorecombinant viruses following exchange of cloned genomic components of the distinct geminiviruses Sida golden mosaic virus (SiGMV-Co) and Abutilon mosaic virus (AbMV) has been investigated. The pseudorecombinant virus produced by DNA A of AbMV and DNA B of SiGMV-Co was infectious in various host plants, although showing a limited host range compared with the two parental viruses. The reciprocal exchange of genomic components did not result in an infectious pseudorecombinant virus. However, in leaf disks SiGMV-Co DNA A was able to trans-replicate the heterogenomic AbMV DNA B component.


Additional keywords: pseudorecombination, trans-replication.

© 1997 The American Phytopathological Society