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Searching for the genetic basis of phenotypic traits of interest of the causal agent of late blight disease through a genome-wide association study
Giovanna Danies: Universidad de los Andes; Laura Gonzalez-Garcia: Universidad de los Andes; Kevin Myers: Cornell University; Howard Judelson: University of California; William Fry: Cornell University; Silvia Restrepo: Universidad de los Andes
<div>Management of <i>Phytophthora infestans</i>, the causal agent of late blight of potatoes and tomatoes has proven to be challenging. This is mainly due to the lack of durable resistance genes and the capability of the pathogen of evolving resistance to certain highly effective fungicides. An understanding of the genetic basis of fungicide resistance would be of value in managing late blight because rapid analysis using molecular markers could inform the selection of the most effective mitigation tactic. In this study, we conducted a genome-wide association study to identify genetic markers associated with sensitivity to mefenoxam in a diverse panel of <i>P. infestans </i>isolates. To reduce the number of contigs in the <i>P. infestans </i>genome, we reassembled it using Pacific Biosciences sequencing data. The closest genes within a 1000 kb flanking region, upstream and downstream, of each significant SNP marker associated with sensitivity to mefenoxam in <i>P. infestans </i>were carefully annotated. Further analyses are needed to confirm these associations.</div>
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