<div>Due to high biocidal properties, copper-based compounds have been utilized to control plant pathogens for centuries. In a previous study, three novel copper nanomaterials (CS-Cu, MV-Cu, and FQ-Cu) were identified as potential alternatives to commercially available copper bactericides for the management of bacterial spot of tomato (<i>Xanthomonas </i>spp.). However, little is known about the potential impact of the copper nanomaterials on the environment. Thus, the objective of this study was to compare the ability of CS-Cu, MV-Cu, FQ-Cu, Kocide^® 3000, and copper-mancozeb to cause changes in bacterial population dynamics in the soil. Soil was collected from a tomato production field in Quincy, FL and divided into 10 cm diameter pots, and incubated at 28°C in a growth chamber. The soil microbiome was investigated by extracting bacterial DNA from soil samples taken at 1 and 7 days post-treatment, and using length-heterogeneity PCR. Non-metric multidimensional scaling analysis of all treatments revealed that the 200 µg/ml concentration of metallic copper from CS-Cu (CS200) at 1 day post-treatment resulted in the greatest shift in the soil microbiome in comparison to the non-treated control. Bacterial amplicon populations from the CS200 treated soil only share 70.7% average similarity with the non-treated control. Although the CS-Cu nanomaterial altered bacterial populations in the soil microbiome, the microbiome reverted to its original state at 7 days post-treatment.</div>