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A Rapid Method for Inoculating Soybean Seedlings with Heterodera glycines. A. P. Rao-Arelli, Department of Agronomy, University of Missouri-Columbia, Delta Center, Portageville 63873. K. W. Matson, and S. C. Anand. Department of Agronomy, University of Missouri-Columbia, Delta Center, Portageville 63873. Plant Dis. 75:594-595. Accepted for publication 17 November 1990. Copyright 1991 The American Phytopathological Society. DOI: 10.1094/PD-75-0594.

A mouth-pipetting method and a newly described method of inoculation of soybean (Glycine max) seedlings with resistance to the soybean cyst nematode (SCN) Heterodera glycines race 3 were evaluated. Neither method resulted in a change for the index of parasitism in soybean genotypes Peking, Pickett-71, PI 90763, or PI 88788. The auto-dispenser method resulted in a narrow range of cysts (160184 per plant) produced on the susceptible cultivar Essex and gave a lower standard of deviation than the mouth-pipetting method. The new method is approximately 18 times faster than the mouth-pipetting method and requires only a single individual to conduct the inoculation. An estimated cost of 94% (dollars) can be saved by adopting the auto-dispenser method.